CDC25A antibody

Catalog No. ABIN264329

The Mouse Monoclonal anti-CDC25A antibody is suitable to detect CDC25A in samples from Human. It has been validated for WB and IP.

Quick Overview for CDC25A antibody (ABIN264329)

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDC25A antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: DCS-121

Product Details anti-CDC25A Antibody

Characteristics: Synonyms: CDC-25A, M-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25A, CellDivision Cycle 25A

Purification: Purified

Immunogen: Purified recombinant CDC25A protein.

Isotype: IgG2a

Application Details

Application Notes: Western Blot. Immunoprecipitation (1-10 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.08 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for CDC25A

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Alternative Name: CDC25A

Background: CDC25A recognizes a 65kD protein In the cell cycle of eukaryotes the M phase is initiated by maturation promoting factor (MPF), a protein kinase complex consisting of p34 cdc2, cyclin B, and p13. The activity of this complex is controlled by multiple levels of phosphorylation and dephosphorylation of the catalytic p34cdc2 component. CDC25A and CDC25B arep34cdc2 tyrosine phosphates. Dephosphorylation of p34cdc2 by these CDC25 proteins activates the MPF complex, initiating mitosis. Although expressed throughout the cell cycle, peak expression of CDC25A is in G1 of the cell cycle.Synonyms: CDC-25A, Cell Division Cycle 25A, Dual specificity phosphatase Cdc25A, M-phase inducer phosphatase 1

Gene ID: 993

UniProt: P30304

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase