LEF1 antibody (HMG Binding Domain)

Catalog No. ABIN264381

The Mouse Monoclonal anti-LEF1 antibody has been validated for WB and IF. It is suitable to detect LEF1 in samples from Human and Mouse.

Quick Overview for LEF1 antibody (HMG Binding Domain) (ABIN264381)

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This LEF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: REMB6

Product Details anti-LEF1 Antibody

Binding Specificity: HMG Binding Domain

Specificity: REMB6 recognizes the High Mobility Group DNA domain of LEF-1.

Characteristics: Synonyms: Lymphoid enhancer-binding factor 1, LEF-1, T cell-specific transcription factor 1-alpha,TCF1-alpha

Purification: Purified

Immunogen: Full-length recombinant LEF-1 protein. Genename: LEF1

Isotype: IgG1

Application Details

Application Notes: Western Blot: 1-5 μg/mLImmunofluorescence on Paraformaldehyde-Fixed Cells: 1 μg/mL (1/1000). Positive Control: Jurkat cell lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS, 0.08 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for LEF1

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Alternative Name: LEF1

Background: Lymphoid Enhancer Factor-1 is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3 and TCF-4). These factors play a crucial role in WNT/WIngless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer.LEF-1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve. It is redundant with TCF-1 (for T Cell Factor-1) for correct development of T lymphocytes in the thymus. LEF-1 is a 399 amino acid protein that appears as a cluster of polypeptides ranging in size between 55-57 kD on SDS-PAGE analysis. Alternative splice forms of LEF-1 and other LEF/TCF family members have been identified, but the expression pattern and relative abundance of these alternative forms have not been well studied. They can appear on westerns as smaller immunoreactive forms.Synonyms: LEF-1, Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, TCF1-alpha

Gene ID: 51176

UniProt: Q9UJU2

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding