CAPN1 antibody
Quick Overview for CAPN1 antibody (ABIN264388)
Target
See all CAPN1 AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Specificity
- This antibody (large subunit, p80) recognizes the 80 kDa subunit of μ-Calpain as well as two smaller proteins that are presumed to be autolysis products.
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Characteristics
- Synonyms: Calpain mu-type, muCANP, CAPN1, CANPL1, PIG30, CANP1, Calpain-1 catalytic subunit,Calpain-1 large subunit, Calcium-activated neutral proteinase 1, Micromolar-calpain, Cellproliferation-inducing gene 30 protein
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Purification
- Standard chromatographic techniques.
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Immunogen
- Calpain purified from human placenta. Genename: CAPN1
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Isotype
- IgG1
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Application Notes
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ELISA: 0.5-1 μg/mL. Western blot: 0.5-1 μg/mL. Immunoprecipitation of both the native and denatured protein: 1-2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Buffer
- 20 mM Sodium Phosphate, pH 7.5, 150 mM Sodium Chloride, 50 % Glycerol and 3 mM Sodium Azide as preservative.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
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Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch. -
Expiry Date
- 12 months
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- CAPN1 (Calpain 1 (CAPN1))
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Alternative Name
- Calpain-1
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Background
- The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and -calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar ( mM) levels of calcium, μ-calpain is active at micromolar (μM) concentrations of Ca++. In addition to the ubiquitously expressed m- and u-calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.Synonyms: CANP1, CANPL1, CAPN1, Calcium-activated neutral proteinase 1, Calpain mu-type, Calpain-1 catalytic subunit, Calpain-1 large subunit, Cell proliferation-inducing gene 30 protein, Micromolar-calpain, PIG30, muCANP
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Gene ID
- 823
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UniProt
- P07384
Target
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