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CAPN1 antibody

This Mouse Monoclonal antibody specifically detects CAPN1 in WB, IP, IHC (p) and EIA. It exhibits reactivity toward Human, Mouse, Rat and Cow.
Catalog No. ABIN264388

Quick Overview for CAPN1 antibody (ABIN264388)

Target

See all CAPN1 Antibodies
CAPN1 (Calpain 1 (CAPN1))

Reactivity

  • 60
  • 36
  • 35
  • 4
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
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Human, Mouse, Rat, Cow

Host

  • 54
  • 15
  • 1
Mouse

Clonality

  • 55
  • 15
Monoclonal

Conjugate

  • 19
  • 12
  • 5
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This CAPN1 antibody is un-conjugated

Application

  • 45
  • 23
  • 19
  • 13
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  • 10
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  • 8
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  • 5
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  • 3
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  • 1
Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone

15C10
  • Specificity

    This antibody (large subunit, p80) recognizes the 80 kDa subunit of μ-Calpain as well as two smaller proteins that are presumed to be autolysis products.

    Characteristics

    Synonyms: Calpain mu-type, muCANP, CAPN1, CANPL1, PIG30, CANP1, Calpain-1 catalytic subunit,Calpain-1 large subunit, Calcium-activated neutral proteinase 1, Micromolar-calpain, Cellproliferation-inducing gene 30 protein

    Purification

    Standard chromatographic techniques.

    Immunogen

    Calpain purified from human placenta. Genename: CAPN1

    Isotype

    IgG1
  • Application Notes

    ELISA: 0.5-1 μg/mL. Western blot: 0.5-1 μg/mL. Immunoprecipitation of both the native and denatured protein: 1-2 μg/mL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Buffer

    20 mM Sodium Phosphate, pH 7.5, 150 mM Sodium Chloride, 50 % Glycerol and 3 mM Sodium Azide as preservative.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Store the antibody at -20 °C. Avoid repeated freezing and thawing.
    Shelf life: One year from despatch.

    Expiry Date

    12 months
  • Target

    CAPN1 (Calpain 1 (CAPN1))

    Alternative Name

    Calpain-1

    Background

    The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and -calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar ( mM) levels of calcium, μ-calpain is active at micromolar (μM) concentrations of Ca++. In addition to the ubiquitously expressed m- and u-calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.Synonyms: CANP1, CANPL1, CAPN1, Calcium-activated neutral proteinase 1, Calpain mu-type, Calpain-1 catalytic subunit, Calpain-1 large subunit, Cell proliferation-inducing gene 30 protein, Micromolar-calpain, PIG30, muCANP

    Gene ID

    823

    UniProt

    P07384
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