CAPN1 antibody

Catalog No. ABIN264388

This Mouse Monoclonal antibody specifically detects CAPN1 in WB, IHC (p), IP and EIA. It exhibits reactivity toward Human, Mouse, Rat and Cow.

Quick Overview for CAPN1 antibody (ABIN264388)

Target: CAPN1 (Calpain 1 (CAPN1))

Reactivity: Human, Mouse, Rat, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This CAPN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Clone: 15C10

Product Details anti-CAPN1 Antibody

Specificity: This antibody (large subunit, p80) recognizes the 80 kDa subunit of μ-Calpain as well as two smaller proteins that are presumed to be autolysis products.

Characteristics: Synonyms: Calpain mu-type, muCANP, CAPN1, CANPL1, PIG30, CANP1, Calpain-1 catalytic subunit,Calpain-1 large subunit, Calcium-activated neutral proteinase 1, Micromolar-calpain, Cellproliferation-inducing gene 30 protein

Purification: Standard chromatographic techniques.

Immunogen: Calpain purified from human placenta. Genename: CAPN1

Isotype: IgG1

Application Details

Application Notes: ELISA: 0.5-1 μg/mL. Western blot: 0.5-1 μg/mL. Immunoprecipitation of both the native and denatured protein: 1-2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: 20 mM Sodium Phosphate, pH 7.5, 150 mM Sodium Chloride, 50 % Glycerol and 3 mM Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for CAPN1

Target: CAPN1 (Calpain 1 (CAPN1))

Alternative Name: Calpain-1

Background: The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and -calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar ( mM) levels of calcium, μ-calpain is active at micromolar (μM) concentrations of Ca++. In addition to the ubiquitously expressed m- and u-calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.Synonyms: CANP1, CANPL1, CAPN1, Calcium-activated neutral proteinase 1, Calpain mu-type, Calpain-1 catalytic subunit, Calpain-1 large subunit, Cell proliferation-inducing gene 30 protein, Micromolar-calpain, PIG30, muCANP

Gene ID: 823

UniProt: P07384