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DNA Adenine Methylase antibody

The Mouse Monoclonal anti-DNA Adenine Methylase antibody (Clone RB1) (ABIN264462) specifically detects DNA Adenine Methylase in WB. The antibody is reactive with Bacteria samples.
Catalog No. ABIN264462
$1,002.00
Plus shipping costs $50.00
0.1 mg
Shipping to: United States
Delivery in 1 to 2 Business Days

Quick Overview for DNA Adenine Methylase antibody (ABIN264462)

Target

DNA Adenine Methylase

Reactivity

Bacteria

Host

  • 1
Mouse

Clonality

  • 1
Monoclonal

Conjugate

  • 1
Un-conjugated

Application

Western Blotting (WB)

Clone

RB1
  • Specificity

    Gram Negative Bacteria.

    Characteristics

    Synonyms: DAM, Deoxyadenosyl-methyltransferase, DNA adenine methyltransferase

    Purification

    Purified

    Immunogen

    Recombinant DNA Adenine Methylase protein.

    Isotype

    IgG1
  • Application Notes

    Western blot (5-10 μg/mL).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    PBS containing 0.08 % Sodium Azide as preservative.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Store the antibody at -20 °C. Avoid repeated freezing and thawing.
    Shelf life: One year from despatch.

    Expiry Date

    12 months
  • Target

    DNA Adenine Methylase

    Background

    DNA adenine methylase (DAM) is an enzyme that methylates adenine residues in the 4-base pair DNA sequence, GATC. This methylation reaction has been shown to be crucial in a variety of cellular processes in bacteria. These include (i) the control of initiation of DNA replication (GATC occurs 11 times in the highly conserved bacterial origin of replication sequence), (ii) methyl-directed mismatch repair, (iii) control of transposition of certain transposable DNA elements, and (iv) control of gene expression. With regard to gene expression, the recent report by Heithoff et al. (Science, 284:967-970, 1999) showed that the deletion of the DAM gene rendered Salmonella typhimurium totally avirulent.Synonyms: DAM, DNA adenine methyltransferase, Deoxyadenosyl-methyltransferase

    Gene ID

    947893

    UniProt

    P0AEE8
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