Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with asynthetic peptide derived from the human TIMP-2 protein and mouse myeloma Ag8563cells. Sequence common in mouse, rabbit, chicken, zebrafish and pig.
TIMP2
Reactivity: Human, Rat, Mouse
IHC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA. Western Blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections: 1 - 5 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0,95 mg/mL
Buffer
PBS, 0.08 % Sodium Azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
TIMP-1, TIMP-2, TIMP-3 and TIMP-4 (for tissue inhibitor of metalloproteinases-1, -2, -3 and -4) complex with metalloproteinases such as collagenases,gelatinases and stromelysins, resulting in irreversible inactivation of the metalloproteinase. TIMP-1 was found to be identical to EPA (erythroidpotentiation activity). Parathyroid hormone has been shown to be a regulator of TIMP-2 in osteoblastic cells. TIMP-3 may be involved in regulating trophoblastic invasion of the uterus as well as in regulating remodeling of the extracellular matrix during the folding of epithelia, and in the formation,branching and expansion of epithelial tubes. TIMP-4 is most highly expressed in heart and low levels of TIMP-4 are expressed in liver, brain, lung, thymus and spleen.Synonyms: CSC-21K, Metalloproteinase inhibitor 2, TIMP-2, Tissue inhibitor of metalloproteinases 2