SERPINC1 antibody

Catalog No. ABIN264614

The Sheep Polyclonal anti-SERPINC1 antibody is suitable to detect SERPINC1 in samples from Human, Mouse, Rat, Dog, Horse and Cat. It has been validated for IHC (p) and ID.

Quick Overview for SERPINC1 antibody (ABIN264614)

Target: SERPINC1 (Serpin Family C Member 1 (SERPINC1))

Reactivity: Human, Mouse, Rat, Dog, Horse, Cat

Host: Sheep

Clonality: Polyclonal

Conjugate: This SERPINC1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunodiffusion (ID)

Product Details anti-SERPINC1 Antibody

Specificity: Antibody to Human Antithrombin III. This Antithrombin III antibody gives a single arc when tested by IEP against human plasma. Identity is confirmed by double diffusion (Ouchterlony) against human plasma and a known anti-human antithrombin III. The cross reactivity of this product against the sera of the species listed below has been assessed by double diffusion using 10 μL of antisera against 5 μL of species sera. These results are intended as a guideline only.

Purification: Ion Exchange Chromatography (DEAE); immunoelectrophoresis (IEP) and double diffusion

Immunogen: Human Antithrombin III purified from plasma, shown to be homogeneous by SDS-PAGE.

Application Details

Application Notes: RID: 6l antiserum / cm2 gel vs.5 μL neat - 1/10 dilution human plasma. Double Diffusion: 10l antiserum vs.5l plasma. IEP: 100l antiserum vs.5l plasma. The use of 3 % PEG 6000 with 1.2 % agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 11.0 mg/mL (OD280nm, E1% = 14.5)

Buffer: Glycine buffered saline, pH 7.4 containing 0.099 % Sodium Azide, 0.1 % EACA, 0.01 % Benzamidine, 1 mM EDTA

Preservative: Sodium azide, Benzamidine, EACA

Precaution of Use: This product contains Sodium azide, Benzamidine and EACA: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for SERPINC1

Target: SERPINC1 (Serpin Family C Member 1 (SERPINC1))

Alternative Name: SERPINC1 / Antithrombin-III

Background: Antithrombin III (ATH III) is a plasma protein synthesised in the liver. Normal plasma levels are 115 to 160 mg/L. ATH III consists of a single polypeptide chain and has a molecular weight of 58 kDa. ATH III is a serine protease inhibitor and regulates coagulation by the formation of covalently liked complexes. ATH III also has a heparin binding site and in the presence of heparin the inhibition of thrombin, factors IXa, Xa and XIa is enhanced 1000 fold. Low levels of ATH III (functional or concentration) are associated with deep vein thrombosis and pulmonary embolism. Antithrombin assays are based on immunologic, enzymatic, or clotting properties.Synonyms: AT3, ATIII, Serpin C1

Gene ID: 462

NCBI Accession: NP_000479

UniProt: P01008