Malondialdehyde antibody (HRP)

Catalog No. ABIN264632

The Goat Polyclonal anti-Malondialdehyde antibody is suitable to detect Malondialdehyde. It has been validated for WB and EIA.

Quick Overview for Malondialdehyde antibody (HRP) (ABIN264632)

Target: Malondialdehyde (MDA)

Host: Goat

Clonality: Polyclonal

Conjugate: This Malondialdehyde antibody is conjugated to HRP

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Malondialdehyde Antibody (HRP)

Purification: MDA modified protein-Sepharose affinity column and conjugated with HRP

Application Details

Application Notes: ELISA. Western Blot: 1: 1,000 - 1: 4,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 0.5 mL distilled water. Centrifuge if not completely clear after standing for 1-2 hours at room temperature.

Concentration: 1.2mg/mL (prior to lyophilization)

Buffer: Lyophilized from 50 mM PBS, 0.1 M Sodium Chloride, pH 7.4 containing 10 mg/mL BSA containing 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Target Details for Malondialdehyde

Target: Malondialdehyde (MDA)

Alternative Name: Malondialdehyde / MDA

Target Type: Chemical

Background: Malondialdehyde (MDA) is a natural product formed in all mammalian cells as a product of lipid peroxidation. MDA is a highly reactive three carbon dialdehyde produced as a byproduct of polyunsaturated fatty acid peroxidation and arachidonic acid metabolism. MDA readily combines with several functional groups on molecules including proteins, lipoproteins, and DNA. It reacts with DNA to form adducts to deoxyguanosine and deoxyadenosine. The major adduct to DNA is a pyrimidopurinone called M1G which appears to be a major endogenous DNA adduct in human beings that may contribute significantly to cancer linked to lifestyle and dietary factors. MDA modified proteins may show altered physico chemical behavior and antigenicity. MDA is toxic and has been implicated in aging mutagenesis, carcinogenesis, diabetic nephropathy and radiation damage. Increased expression of MDA has been reported in the brains of Alzheimer's patients. Antibodies to MDA will help to visualize the MDA adducts.Synonyms: 1, 3-Propanedial, 3-Propanedione, Malondialdehyde Malonyldialdehyde, Malonic aldehyde, Malonic dialdehyde, Malonodialdehyde, Propanedial