Uromodulin antibody
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- Target See all Uromodulin (UMOD) Antibodies
- Uromodulin (UMOD)
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Reactivity
- Human
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Host
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Sheep
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Clonality
- Polyclonal
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Conjugate
- This Uromodulin antibody is un-conjugated
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Application
- Immunodiffusion (ID)
- Specificity
- This antibody gives a single arc when tested by IEP against fresh urine. No arcs are visible when tested by IEP against serum. Identity has been confirmed by double diffusion (Ouchterlony) against uromucoid and a known anti-uromucoid.
- Purification
- Ion exchange chromatography
- Immunogen
- Human Uromucoid, purified from human urine
- Top Product
- Discover our top product UMOD Primary Antibody
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- Application Notes
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The use of 3 % PEG 6000 with 1.2 % agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended. RID: 10l antiserum/cm2 in gel vs 5l uromucoid - 50 mg/L Neat - 1: 5. Double Diffusion: 10l antiserum vs 3l uromucoid - 50 mg/LIEP: 100l antiserum vs 5 μL uromucoid - 50 mg/L
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 14.8mg/mL (OD280nm E1% = 14.5)
- Buffer
- Glycine buffered saline, pH 7.4 containing 0.09 % Sodium azide, 0.1 % E-Amino-n-Caproic acid, 0.01 % Benzamidine, 1 mM EDTA
- Preservative
- Sodium azide, Benzamidine, EACA
- Precaution of Use
- This product contains Sodium azide, Benzamidine and EACA: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C.
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- Target
- Uromodulin (UMOD)
- Abstract
- UMOD Products
- Synonyms
- ADMCKD2 antibody, FJHN antibody, HNFJ antibody, HNFJ1 antibody, MCKD2 antibody, THGP antibody, THP antibody, urehr4 antibody, uromodulin antibody, UMOD antibody, Umod antibody
- Background
- Synonyms: THP, Tamm-Horsfall urinary glycoprotein
- Gene ID
- 7369
- NCBI Accession
- NP_001008390
- UniProt
- P07911
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