BrdU antibody

Catalog No. ABIN264907

This Sheep Polyclonal antibody specifically detects BrdU in IHC (p), WB and IP.

Quick Overview for BrdU antibody (ABIN264907)

Target: BrdU (Bromodeoxyuridine (BrdU))

Host: Sheep

Clonality: Polyclonal

Conjugate: This BrdU antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-BrdU Antibody

Purification: Protein G purified

Isotype: IgG

Application Details

Application Notes: Western blotting: 25 - 100 μg/mL. Immunoprecipitation: 25 - 100 μg/mL. Immunohistochemistry: 25 - 100 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS, 0.08 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Target Type: Chemical

Background: Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Previous methods of detecting DNA included the use of [3H]-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting. These methods are more difficult and require more cleanup due to the radioactive material. An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells.