ADAM10 antibody (AA 732-748)

Catalog No. ABIN264988

This Rabbit Polyclonal antibody specifically detects ADAM10 in WB. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for ADAM10 antibody (AA 732-748) (ABIN264988)

Target: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADAM10 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ADAM10 Antibody

Binding Specificity: AA 732-748

Specificity: This antibody recognizes ADAM10.

Purification: Purified

Immunogen: Synthetic peptide corresponding to amino acids 732-748 off the Human ADAM10.

Isotype: IgG

Application Details

Application Notes: Western Blot: 0.25-1 μg/mL. Detects an 85 kDa band can be detected in Jurkat cell lysates which may representprecursor protein. A faint 60 kDa band is also detected in some cell lines, including Jurkat,which may be processed mature protein. Positive Control/Tissue Expression: Jurkat whole cell lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline solution containing 0.02 % Sodium Azide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C.

Target Details for ADAM10

Target: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Alternative Name: CD156c / ADAM10

Background: Proinflammatory cytokine tumor necrosis factor-a (TNF-a) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-a, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-a, notch and its ligand delta (1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively, (1,2,4). ADAM10 mRNA is expressed in a variety of human and bovine tissues (1,4).Synonyms: Disintegrin and metalloproteinase domain-containing protein 10, KUZ, Kuzbanian protein homolog, MADM, Mammalian disintegrin-metalloprotease

Gene ID: 102

NCBI Accession: NP_001101

UniProt: O14672

Pathways: Notch Signaling, EGFR Signaling Pathway