BUB1B antibody (C-Term)

Catalog No. ABIN265006

This Sheep Polyclonal antibody specifically detects BUB1B in WB and IHC (fro). It exhibits reactivity toward Mouse.

Quick Overview for BUB1B antibody (C-Term) (ABIN265006)

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Reactivity: Mouse

Host: Sheep

Clonality: Polyclonal

Conjugate: This BUB1B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-BUB1B Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts to Bub-1b (C-term).

Purification: Purified

Immunogen: Recombinant protein corresponding to amino acids 883 - 1052 of the mouse Bub1b protein.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1 - 10 μg/mL, Detects a 130 kDa band. POSITIVE CONTROL: Mitotic NIH/3T3 cells. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only.

Target Details for BUB1B

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Alternative Name: BUB1B

Background: Bub1, a mitotic checkpoint gene first identified in yeast, ensures proper chromosome segregation by delaying anaphase until chromosomes are properly aligned on the mitotic spindle. Mutations in the mitotic spindle assembly checkpoint genes, like yeast Bub1, cause cells to exit mitosis without proper chromosome attachment/alignment, resulting in aneuploidy. Aneuploidy is a form of genetic instability involved in cancer. Recently, a novel homologue of the murine Bub1 gene has been identified and named Bub1b. The yeast mitotic checkpoint pathway is unresolved, but the mammalian pathway remains even less clear. It is important to investigate the role of the Bub1 genes in the mammalian checkpoint as they have been implicated in carcinogenesis, namely colon carcinomas. The aneuploidy and chromosome instability characterizing some tumors is thought to be caused by mutations in Bub1.Synonyms: BUB1 beta, BUBR1, MAD3/BUB1-related protein kinase, MAD3L, Mitotic checkpoint kinase MAD3L, Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, Protein SSK1

Gene ID: 701

NCBI Accession: NP_001202

UniProt: O60566