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ASK1 antibody

MAP3K5 Reactivity: Human, Mouse WB, IHC (p), EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN265316
  • Target See all ASK1 (MAP3K5) Antibodies
    ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))
    Reactivity
    • 183
    • 100
    • 57
    • 18
    • 15
    • 8
    Human, Mouse
    Host
    • 192
    • 8
    Rabbit
    Clonality
    • 172
    • 28
    Polyclonal
    Conjugate
    • 82
    • 12
    • 12
    • 9
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 1
    This ASK1 antibody is un-conjugated
    Application
    • 159
    • 58
    • 39
    • 39
    • 38
    • 30
    • 29
    • 20
    • 11
    • 11
    • 7
    • 4
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    Specificity
    This antibody detects endogenous levels of MEKK5 / ASK1 protein(region surrounding Glu960).
    Cross-Reactivity (Details)
    Species reactivity (expected):Mouse and Rat.
    Species reactivity (tested):Human.
    Purification
    Affinity chromatography using epitope-specific immunogen.
    Top Product
    Discover our top product MAP3K5 Primary Antibody
  • Application Notes
    ELISA: 1/20000approx. 1/40000. Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))
    Alternative Name
    MEKK5 / ASK1 (MAP3K5 Products)
    Synonyms
    ASK1 antibody, MAPKKK5 antibody, MEKK5 antibody, mitogen-activated protein kinase kinase kinase 5 antibody, MUD21.11 antibody, MUD21_11 antibody, 7420452D20Rik antibody, ASK antibody, Mekk5 antibody, Ask1 antibody, RGD1306565 antibody, mitogen-activated protein kinase kinase kinase 5 antibody, MAP3K5 antibody, MAPKKK5 antibody, Map3k5 antibody, map3k5 antibody
    Background
    Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also called MAP kinase kinase kinases) phosphorylate and activate the MAP kinases, including ERK, JNK and p38. The MEK kinases characterized to date include Raf-1, Raf-B, MOS, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4 and ASK 1 (also designated MEK kinase-5). MEK kinase-1 has been shown to phosphorylate MEK-1 via a Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK pathway. ASK1 activates both MEK-4 and MEK-3/MEK-6 pathways.Synonyms: Apoptosis signal-regulating kinase 1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, Mitogen-activated protein kinase kinase kinase 5
    Molecular Weight
    approx. 155 kDa
    Gene ID
    4217
    NCBI Accession
    NP_005914
    UniProt
    Q99683
    Pathways
    MAPK Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response
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