Caveolin-1 antibody

Catalog No. ABIN265338

The Rabbit Polyclonal anti-Caveolin-1 antibody (ABIN265338) specifically detects Caveolin-1 in WB, IF and EIA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Caveolin-1 antibody (ABIN265338)

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caveolin-1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-Caveolin-1 Antibody

Specificity: This antibody detects endogenous levels of Caveolin-1 protein (region surrounding Val163).

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 5000 approx. 1: 10000. WB: 1: 500approx. 1: 1000. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Caveolin-1

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Alternative Name: Caveolin-1

Background: Caveolae are specialised domains of the plasma membrane that are implicated in the sequestration of a variety of lipid and protein molecules. It has been suggested that these important cellular organelles have a pivotal role in such diverse biochemical processes as lipid metabolism, growth regulation, signal transduction, and apoptosis. Caveolin interacts with and regulates heterotrimeric G proteins. Currently, there are three members of the caveolin multigene family which are known to encode 21 to 24 kDa integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. Caveolin 2 protein is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells. The expression of caveolin 1 is similar to that of caveolin 2 while caveolin 3 expression appears to be limited to muscle tissue types.Synonyms: CAV1

Molecular Weight: approx. 25 kDa

Gene ID: 857

NCBI Accession: NP_001744

UniProt: Q03135

Pathways: Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of Transporter Activity, VEGFR1 Specific Signals