Crk antibody

Catalog No. ABIN265373

Product Details anti-Crk Antibody

Target: Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Crk antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects endogenous levels of CRK protein (region surrounding aa215).

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Crk

Target: Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))

Alternative Name: CRK (CRK Products)

Synonyms: CG1587 antibody, CRK antibody, D-CRK antibody, D-Crk antibody, DCrk antibody, Dcrk antibody, Dmel\\CG1587 antibody, crk antibody, dCRK antibody, dCrk antibody, fb34h10 antibody, fc05a01 antibody, fi19g05 antibody, wu:fb34h10 antibody, wu:fc05a01 antibody, wu:fc54a04 antibody, wu:fi19g05 antibody, zgc:100936 antibody, CRKII antibody, p38 antibody, Crko antibody, crk2 antibody, crkII antibody, v-crk antibody, P38C-CRK antibody, Crk-I antibody, Crk-II antibody, Crk-III antibody, Crk3 antibody, CrkIII antibody, c-Crk antibody, Crk oncogene antibody, v-crk avian sarcoma virus CT10 oncogene homolog antibody, CRK proto-oncogene, adaptor protein antibody, v-crk avian sarcoma virus CT10 oncogene homolog L homeolog antibody, v-crk sarcoma virus CT10 oncogene homolog antibody, Crk antibody, crk antibody, CRK antibody, crk.L antibody

Background: Crk I and Crk II are encoded by the same gene, which undergoes alternative splicing to yield these two proteins, but differ in their biological activities. Crk II has less transforming activity than Crk I, although both Crk I and Crk II bind to many tyrosine-phosphorylated proteins that bind to GRB2. In addition, Crk II becomes rapidly tyrosine-phosphorylated in response to stimulation with insulin-like growth factor I (IGF-I) and might be involved in the IGF-I receptor signalling pathway.Synonyms: Adapter molecule crk, Proto-oncogene C-crk, p38

Molecular Weight: approx. 42 kDa

Gene ID: 1398

NCBI Accession: NP_005197

UniProt: P46108

Pathways: Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor