DP1 antibody

Catalog No. ABIN265387

This Rabbit Polyclonal antibody specifically detects DP1 in WB, IF, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for DP1 antibody (ABIN265387)

Target: DP1 (TFDP1) (Transcription Factor Dp-1 (TFDP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-DP1 Antibody

Specificity: This antibody detects endogenous levels of DP-1/TFDP1 protein (region surrounding Val393).

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 5000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DP1

Target: DP1 (TFDP1) (Transcription Factor Dp-1 (TFDP1))

Alternative Name: TFDP1

Background: The human retinoblastoma gene product appears to play an important role in the negative egulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 forms heterodimers with a second protein, designated DP-1, forming an Synonyms: DP1, DRTF1, DRTF1-polypeptide 1, E2F dimerization partner 1, Transcription factor Dp-1

Molecular Weight: approx. 55 kDa

Gene ID: 7027

NCBI Accession: NP_009042

UniProt: Q14186

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases