EEF2 antibody
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- Target See all EEF2 Antibodies
- EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This EEF2 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- This antibody detects endogenous levels of eEF2 protein. (region surrounding Ala50)
- Cross-Reactivity (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
- Purity
- > 95 % pure by SDS-PAGE
- Top Product
- Discover our top product EEF2 Primary Antibody
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- Application Notes
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ELISA: 1: 5000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))
- Alternative Name
- EEF2 (EEF2 Products)
- Synonyms
- EEF-2 antibody, EF-2 antibody, EF2 antibody, Ef-2 antibody, eef2 antibody, MGC76191 antibody, MGC79628 antibody, EEF2 antibody, eef2l antibody, fe49h02 antibody, wu:fe49h02 antibody, zgc:63584 antibody, ef2 antibody, zef2 antibody, CG2238 antibody, Dmel\CG2238 antibody, EF-2b antibody, EF2B antibody, EF2b antibody, Ef2 antibody, Ef2B antibody, Ef2b antibody, anon-EST:Liang-1.44 antibody, chr2L:21668915..21669164 antibody, clone 1.44 antibody, eEF2 antibody, eF2 antibody, ef2b antibody, eukaryotic translation elongation factor 2 antibody, eukaryotic translation elongation factor 2, gene 1 antibody, eukaryotic translation elongation factor 2b antibody, Elongation factor 2 antibody, EEF2 antibody, Eef2 antibody, eef2.1 antibody, POSPLDRAFT_118836 antibody, eef2b antibody, eef-2 antibody, eef2 antibody, EF2 antibody
- Background
- Two elongation factors (EF) EF-Tu and EF-2 participate in the elongation phase during protein biosynthesis on the ribosome and their functional cycles depend on GTP binding and its hydrolysis. EF-Tu (also designated mitochondrial precursor p43) and EF-2 are multidomain GTPases with essential functions in translation, and they both bind to the same site on the ribosome where their low intrinsic GTPase activities are strongly stimulated. EF-Tu plays a central role in the fast and accurate delivery of aminoacyl-tRNAs to the translating ribosome. In addition, EF-Tu protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anticodon on tRNA can be achieved.Synonyms: EF-2, EF2, Elongation factor 2
- Molecular Weight
- approx. 100 kDa
- Gene ID
- 1938
- NCBI Accession
- NP_001952
- UniProt
- P13639
- Pathways
- AMPK Signaling
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