MMP2 antibody

Catalog No. ABIN265529

The Rabbit Polyclonal anti-MMP2 antibody has been validated for WB and EIA. It is suitable to detect MMP2 in samples from Human, Rat and Mouse.

Quick Overview for MMP2 antibody (ABIN265529)

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-MMP2 Antibody

Specificity: This antibody detects endogenous levels of MMP-2 protein. (region surrounding Leu638)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 60000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for MMP2

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Alternative Name: MMP-2

Background: The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-2 (also designated type IV collagenase) cleaves collagen types IV, V, VII and X and gelatin type I. Activation of MMP-2 secretion requires the Ras signaling pathway.Synonyms: 72 kDa gelatinase, 72 kDa type IV collagenase, CLG4A, Gelatinase A, MMP2, Matrix metalloproteinase-2, TBE-1

Molecular Weight: approx. 74 kDa

Gene ID: 4313

NCBI Accession: NP_001121363

UniProt: P08253

Pathways: Activation of Innate immune Response