alpha Tubulin antibody (Alexa Fluor 488)

Catalog No. ABIN2656795

The Mouse Monoclonal anti-alpha Tubulin antibody has been validated for FACS. It is suitable to detect alpha Tubulin in samples from Human, Mouse and Rat.

Quick Overview for alpha Tubulin antibody (Alexa Fluor 488) (ABIN2656795)

Target: alpha Tubulin (TUBA1)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha Tubulin antibody is conjugated to Alexa Fluor 488

Application: Flow Cytometry (FACS)

Clone: 10D8

Product Details anti-alpha Tubulin Antibody (Alexa Fluor 488)

Purification: The antibody was conjugated with Alexa Fluor® 488 under optimal conditions.

Purity: The antibody is at > 85 % purity.

Isotype: IgM kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: phosphate-buffered solution, pH 7.4, containing 0.09 % sodium azide at 0.5 mg/mL.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C

Target Details for alpha Tubulin

Target: alpha Tubulin (TUBA1)

Alternative Name: Tubulin-alpha

Background: Tubulin, one of the major constituents of microtubules, is a dimeric protein consisting of an alpha and beta chain. Tubulin is a highly conserved protein that is ubiquitously expressed. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation. Tubulin interacts with a variety of proteins including RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, and microtubule associated protein 1A. The 10D8 antibody recognizes α-tubulin in all species and is useful for Western blotting, and immunofluorescence staining. The 10D8 antibody can also be used to monitor protein loading in Western blotting.

Pathways: Microtubule Dynamics