I-AD antibody (Alexa Fluor 488)

Catalog No. ABIN2657527

The Mouse Monoclonal anti-I-AD antibody is suitable to detect I-AD in samples from Mouse. It has been validated for FACS, WB, ICC and IP.

Quick Overview for I-AD antibody (Alexa Fluor 488) (ABIN2657527)

Target: I-AD

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Alexa Fluor 488

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: 39-10-8

Product Details

Cross-Reactivity (Details): Mouse d and g7 haplotypes, Does not cross-react with other haplotypes ( e.g., a, b, k, p, q, s)

Characteristics: The 39-10-8 antibody reacts with the I-Ad MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2d bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 39-10-8 antibody does not cross-react with other haplotypes (a, b, k, p, q, s), but has been demonstrated to cross-react with the g7 haplotype.

Purification: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions.

Isotype: IgG3 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C

Target Details

Target: I-AD