CD14 antibody (Alexa Fluor 647)

Catalog No. ABIN2657631

This Mouse Monoclonal antibody specifically detects CD14 in FACS. It exhibits reactivity toward Human.

Quick Overview for CD14 antibody (Alexa Fluor 647) (ABIN2657631)

Target: CD14

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD14 antibody is conjugated to Alexa Fluor 647

Application: Flow Cytometry (FACS)

Clone: M5E2

Product Details anti-CD14 Antibody (Alexa Fluor 647)

Cross-Reactivity: Chimpanzee, Cynomolgus, Rhesus Monkey, Capuchin Monkey, Saguinus oedipus, Pigtailed Macaque, Marmoset, Squirrel Monkey (Saimiri spec.), Cow (Bovine), Pig (Porcine), Dog (Canine)

Purification: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C

Target Details for CD14

Target: CD14

Alternative Name: CD14

Background: CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens, and in the upregulation of adhesion molecules and expression of cytokines in monocytes and neutrophils.

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades