CD25 antibody (Alexa Fluor 647)

Catalog No. ABIN2657710

The Rat Monoclonal anti-CD25 antibody has been validated for FACS and CyTOF. It is suitable to detect CD25 in samples from Mouse.

Quick Overview for CD25 antibody (Alexa Fluor 647) (ABIN2657710)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD25 antibody is conjugated to Alexa Fluor 647

Application: Flow Cytometry (FACS), Cytometry by Time of Flight (CyTOF)

Clone: PC61

Product Details anti-CD25 Antibody (Alexa Fluor 647)

Purification: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.

Isotype: IgG1 lambda

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: CD25

Background: CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation