ITGA5 antibody (Alexa Fluor 647)

Catalog No. ABIN2657814

This Rat Monoclonal antibody specifically detects ITGA5 in FACS and IHC. It exhibits reactivity toward Mouse.

Quick Overview for ITGA5 antibody (Alexa Fluor 647) (ABIN2657814)

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This ITGA5 antibody is conjugated to Alexa Fluor 647

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Clone: 5H10-27(MFR5)

Product Details anti-ITGA5 Antibody (Alexa Fluor 647)

Purification: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C

Target Details for ITGA5

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Alternative Name: CD49e

Background: CD49e is a 135 kD protein, also known as α5 integrin or VLA-5 α chain. It is a member of the integrin family, expressed on thymocytes, splenic B cells, activated T cells, and mast cells. CD49e associates with CD29 (integrin β1 chain) to form the fibronectin receptor (VLA-5). CD49e plays a critical role in both adhesion and T cell costimulation. The primary ligand for CD49e/CD29 (VLA-5) is fibronectin. The 5H10-27(MFR5) antibody has been shown to block CD49e mediated interactions and promote the in vitro stimulation of CD8+ T cells.

Pathways: Integrin Complex