CD80 antibody (Alexa Fluor 647)

Catalog No. ABIN2657845

The Hamster Monoclonal anti-CD80 antibody has been validated for FACS, IHC and CyTOF. It is suitable to detect CD80 in samples from Mouse.

Quick Overview for CD80 antibody (Alexa Fluor 647) (ABIN2657845)

Target: CD80

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD80 antibody is conjugated to Alexa Fluor 647

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Cytometry by Time of Flight (CyTOF)

Clone: 16-10A1

Product Details anti-CD80 Antibody (Alexa Fluor 647)

Cross-Reactivity: Dog (Canine)

Purification: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C

Target Details for CD80

Target: CD80

Alternative Name: CD80

Background: CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints