ESAM antibody (APC)

Catalog No. ABIN2658754

The Rat Monoclonal anti-ESAM antibody is suitable to detect ESAM in samples from Mouse. It has been validated for IF and FACS.

Quick Overview for ESAM antibody (APC) (ABIN2658754)

Target: ESAM (Endothelial Cell Adhesion Molecule (ESAM))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This ESAM antibody is conjugated to APC

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 1G8-ESAM

Product Details anti-ESAM Antibody (APC)

Purification: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for ESAM

Target: ESAM (Endothelial Cell Adhesion Molecule (ESAM))

Alternative Name: ESAM

Background: Endothelial cell-selective adhesion molecule (ESAM) is a 55-kD membrane protein composed of two extracellular Ig domains, a single transmembrane domain, and a cytoplasmic domain. ESAM is predominantly expressed at endothelial junctions and on platelets, participating in the migration of neutrophils through the vessel wall by influencing endothelial cell contacts. It impacts vascular permeability and extravasation process. Recently, it was reported that ESAM is a novel marker for murine hematopoietic stem cells (HSCs) in fetal liver. ESAM expression is correlated with HSC activity. The ESAMHi population was highly enriched for multipotent myeloid-erythroid progenitors and primitive progenitors with lymphpoietic activity, and exclusively reconstituted long-term lymphohematopoiesis in lethally irradiated recipients.