C5 antibody (Biotin)

Catalog No. ABIN2660802

The Mouse Monoclonal anti-C5 antibody has been validated for ICFC. It is suitable to detect C5 in samples from Human.

Quick Overview for C5 antibody (Biotin) (ABIN2660802)

Target: C5 (Complement Component 5 (C5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This C5 antibody is conjugated to Biotin

Application: Intracellular Flow Cytometry (ICFC)

Clone: G25-2

Product Details anti-C5 Antibody (Biotin)

Purification: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for C5

Target: C5 (Complement Component 5 (C5))

Alternative Name: C5a/C5a/C5

Background: C5, complement component 5, is a protein involved in the complement system. It is cleaved into C5a and C5b by the C3/C5 convertase. The smaller fragment C5a is an anaphylatoxin and is released into the fluid surroundings. C5a is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis). C5a contains 74 amino acids and is rapidly metabolised by a serum enzyme, carboxypeptidase B to a 73 amino acid form, C5a des-Arg. The major fragment C5b binds to the membrane initiating the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex.

Pathways: Complement System, Carbohydrate Homeostasis