BrdU antibody (Biotin)

Catalog No. ABIN2660870

The Biotin-conjugated Mouse Monoclonal anti-BrdU antibody (Clone MoBU-1) (ABIN2660870) specifically detects BrdU in IHC, FACS and IF.

Quick Overview for BrdU antibody (Biotin) (ABIN2660870)

Target: BrdU (Bromodeoxyuridine (BrdU))

Host: Mouse

Clonality: Monoclonal

Conjugate: This BrdU antibody is conjugated to Biotin

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: MoBU-1

Product Details anti-BrdU Antibody (Biotin)

Purification: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Alternative Name: BrdU

Target Type: Chemical

Background: BrdU is a uridine derivative and a structural analog of thymidine that can be incorporated into DNA during the S-phase of the cell cycle as a substitute for thymidine. Cells can be pulse-labeled with BrdU and analyzed with antibodies against BrdU to determine the proportion of cells in the S-phase of the cell cycle during a given interval. The MoBU-1 antibody is useful for the detection of incorporated BrdU by fluorescent cell staining (ICFC), flow cytometry, and immunohistochemistry.