ITGA4 antibody (FITC)

Catalog No. ABIN2661757

The Mouse Monoclonal anti-ITGA4 antibody is suitable to detect ITGA4 in samples from Rat. It has been validated for FACS, IHC and IF.

Quick Overview for ITGA4 antibody (FITC) (ABIN2661757)

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGA4 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: MRalpha4-1

Product Details anti-ITGA4 Antibody (FITC)

Purification: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Alternative Name: CD49d

Background: CD49d is a 150 kD glycoprotein, also known as integrin α4 or VLA-4 α chain. It is expressed as a heterodimer with either of two β chains, β1 or β7. The α4β1 integrin (also known as VLA-4, CD49d/CD29) is expressed on peripheral T and B lymphocytes, thymocytes, and monocytes, while the α4β7 integrin (also known as LPAM-1) is expressed on peripheral lymphocytes. The α4β1 and α4β7 integrins mediate cell-cell and cell-matrix interactions by interacting with the ligands CD106 (VCAM-1), MAdCAM-1, and fibronectin. Soluble MRα4-1 antibody can partially inhibit in vitro binding of VCAM-1 and mast cells to fibronectin and inhibit mast cell degranulation. Immobilized MRα4-1 antibody can enhance mast cell degranulation in vitro.

Pathways: Integrin Complex