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I-AD antibody (FITC)

The FITC-conjugated Mouse Monoclonal anti-I-AD antibody (Clone 39-10-8) (ABIN2661925) specifically detects I-AD in WB, ELISA (Detection) and ELISPOT (Detection). The antibody is reactive with Mouse samples.
Catalog No. ABIN2661925
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Quick Overview for I-AD antibody (FITC) (ABIN2661925)

Target

I-AD

Reactivity

Mouse

Host

  • 1
Mouse

Clonality

  • 1
Monoclonal

Conjugate

  • 1
FITC

Application

Western Blotting (WB), ELISA (Detection), ELISpot (Detection) (ELISPOT (Detection))

Clone

39-10-8
  • Cross-Reactivity (Details)

    Mouse d and g7 haplotype, Does not cross-react with other haplotypes ( e.g., a, b, k, p, q, s)

    Characteristics

    The 39-10-8 antibody reacts with the I-Ad MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2d bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 39-10-8 antibody does not cross-react with other haplotypes (a, b, k, p, q, s), but has been demonstrated to cross-react with the g7 haplotype.

    Purification

    The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.

    Isotype

    IgG3 kappa
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Concentration

    0.5 mg/mL

    Buffer

    Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Protect from prolonged exposure to light. Do not freeze.

    Storage

    4 °C

    Storage Comment

    The antibody solution should be stored undiluted between 2°C and 8°C.
  • Target

    I-AD
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