T-Bet antibody (Pacific Blue)

Catalog No. ABIN2662108

The Mouse Monoclonal anti-T-Bet antibody has been validated for FACS. It is suitable to detect T-Bet in samples from Human and Mouse.

Quick Overview for T-Bet antibody (Pacific Blue) (ABIN2662108)

Target: T-Bet

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This T-Bet antibody is conjugated to Pacific Blue

Application: Flow Cytometry (FACS)

Clone: 4B10

Product Details anti-T-Bet Antibody (Pacific Blue)

Purification: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ .

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for T-Bet

Target: T-Bet

Background: T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.