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ISM1 antibody (PE)

ISM1 Reactivity: Mouse FACS Host: Rat Monoclonal B054B6 PE
Catalog No. ABIN2662774
  • Target See all ISM1 Antibodies
    ISM1 (Isthmin 1 (ISM1))
    Reactivity
    • 24
    • 17
    Mouse
    Host
    • 24
    Rat
    Clonality
    • 24
    Monoclonal
    Conjugate
    • 7
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ISM1 antibody is conjugated to PE
    Application
    • 16
    • 14
    • 13
    • 3
    Flow Cytometry (FACS)
    Purification
    The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
    Clone
    B054B6
    Isotype
    IgG2a kappa
    Top Product
    Discover our top product ISM1 Primary Antibody
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    0.2 mg/mL
    Buffer
    Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect from prolonged exposure to light. Do not freeze.
    Storage
    4 °C
    Storage Comment
    The antibody solution should be stored undiluted between 2°C and 8°C.
  • Target
    ISM1 (Isthmin 1 (ISM1))
    Alternative Name
    Isthmin-1 (ISM1 Products)
    Synonyms
    5430433G21Rik antibody, Ism antibody, C20orf82 antibody, ISM antibody, Isthmin antibody, bA149I18.1 antibody, dJ1077I2.1 antibody, ism antibody, xIsm antibody, isthmin 1 antibody, isthmin 1, angiogenesis inhibitor antibody, isthmin 1, angiogenesis inhibitor L homeolog antibody, ism1 antibody, Ism1 antibody, ISM1 antibody, ism1.L antibody
    Background
    Isthmin is a secreted protein, and it was initially identified in the Xenopus midbrain-hindbrain organizer (MHB) or isthmus organizer, where it is highly expressed. The MHB is an important signaling center in vertebrates and regulates the polarized morphological differentiation of the adjacent tectum and cerebellum. Mouse Isthmin is a 454 amino acid protein containing a Thrombospondin Type 1 Repeat (TSR) domain in the central region and an Adhesion-associated domain in MUC4 and Other Proteins (AMOP) domain at the C-terminal. The TSR domains are highly conserved with 98 % identity between mouse and human, 87-88 % identity between mouse and Xenopus. The C-terminal AMOP domains are also highly conserved with 99 % identity between mouse and human, 91 % identity between mouse and Xenopus. Mouse isthmin nucleotide sequence is similar to the human chromosome 20 open reading frame 82 (C20orf82). The Ism1 gene is conserved in human, chimpanzee, dog, cow, chicken, and zebrafish. Isthmin has angiostatic activity in vitro and in vivo. Isthmin inhibits EC capillary network formation mainly by interfering with the early stages of in vitro angiogenesis on matrigel. Isthmin inhibits VEGF-induced EC proliferation and induces apoptosis in the presence of VEGF. Overexpression of isthmin in B16 melanoma inhibits tumor growth and tumor angiogenesis in mice. Knockdown of isthmin in zebrafish embryos leads to abnormal intersegmental vessel (ISV) formation in the trunk.
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