IL3RA antibody (PE)

Catalog No. ABIN2662992

This Rat Monoclonal antibody specifically detects IL3RA in ICFC and ELISA (Detection). It exhibits reactivity toward Mouse.

Quick Overview for IL3RA antibody (PE) (ABIN2662992)

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL3RA antibody is conjugated to PE

Application: Intracellular Flow Cytometry (ICFC), ELISA (Detection)

Clone: 5B11

Product Details anti-IL3RA Antibody (PE)

Purification: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for IL3RA

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Alternative Name: CD123

Background: CD123 is a 70 kD α chain subunit of the IL-3 receptor (IL-3R α). It is a member of the immunoglobulin superfamily that is expressed on hematopoietic progenitors, basophils, mast cells, and megakaryocytes. This transmembrane glycoprotein can bind IL-3 with low affinity but cannot transduce signals without association with additional protein partners. CD123 can complex with either the common β chain (CDw131) or the IL-3R β chain (AIC2A) to form high-affinity heterodimeric IL-3 receptors. CDw131 can complex with the α subunits of the mouse IL-3R, IL-5R and GM-CSFR to form high-affinity receptors, while the IL-3 R β subunit is specific for IL-3 but binds with low affinity. IL-3 binding to the receptor complex can induce proliferation and differentiation of hematopoietic cells. The 5B11 antibody does not block binding of IL-3 to the high affinity IL-3 receptor.

Pathways: JAK-STAT Signaling