C5AR1 antibody (PE)

Catalog No. ABIN2663629

This Rat Monoclonal antibody specifically detects C5AR1 in FACS, IHC and IF. It exhibits reactivity toward Mouse.

Quick Overview for C5AR1 antibody (PE) (ABIN2663629)

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This C5AR1 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: 20-70

Product Details anti-C5AR1 Antibody (PE)

Purification: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for C5AR1

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Alternative Name: CD88

Background: CD88 is a single chain protein with seven membrane-spanning regions and a molecular weight of 43 kD. CD88 is expressed by neutrophils, eosinophils, and monocytes. There have been reports of CD88 expression in non-immune cells such as glial cells, cerebellar granule cells, cardiomyocytes, and vascular endothelial cells. CD88 is coupled to heterotrimeric G proteins such as Gi, and after C5a binding, the signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA2, among other molecules, resulting in chemotaxis, granule enzyme release, and superoxide anion production.

Pathways: Complement System