Histone 3 antibody (pSer28)

Catalog No. ABIN2666077

The Rat Monoclonal anti-Histone 3 antibody is suitable to detect Histone 3 in samples from Human. It has been validated for ICFC.

Quick Overview for Histone 3 antibody (pSer28) (ABIN2666077)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Intracellular Flow Cytometry (ICFC)

Clone: HTA28

Product Details anti-Histone 3 Antibody

Binding Specificity: pSer28

Purification: The antibody was purified by affinity chromatography.

Immunogen: AA 23-35 of human histone H3.

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide. Final antibody concentration is 0.5 mg/mL.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Upon receipt, store between 2°C and 8°C.

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background: H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability. During mitosis, H3 is phosphorylated at serine 28.This phosphorylation coincides with chromosome condensation initiated at prophase and disappears at late anaphase. H3 has been demonstrated to be phosphorylated by the action of MLTK-α (mixed linage kinase-like mitogen activated protein triple kinase α) in response to ultraviolet B light and epidermal growth factor, as well as Aurora-B during mitosis.