Histone H1 antibody

Catalog No. ABIN2667975

This Rabbit Polyclonal antibody specifically detects Histone H1 in WB, IF and ICC. It exhibits reactivity toward Human. It has been mentioned in 2+ publications

Quick Overview for Histone H1 antibody (ABIN2667975)

Target: Histone H1

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Histone H1 Antibody

Purpose: Histone H1 antibody (pAb)

Purification: Unpurified

Immunogen: This Histone H1 antibody was raised against calf thymus histone H1.

Application Details

Application Notes: ICC/IF: 1:500 - 1:1,000 dilution WB: 1:500 - 1:5,000 dilution

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Histone H1 antibody (ABIN2667975)

Li, Wu, Kim, Zhao, Hearn, Zhang, Meistrich, Mills: "Chd5 orchestrates chromatin remodelling during sperm development." in: Nature communications, Vol. 5, pp. 3812, (2014) (PubMed).

Hagarman, Motley, Kristjansdottir, Soloway: "Coordinate regulation of DNA methylation and H3K27me3 in mouse embryonic stem cells." in: PLoS ONE, Vol. 8, Issue 1, pp. e53880, (2013) (PubMed).

Target Details for Histone H1

Target: Histone H1

Background: The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression.

Molecular Weight: 31 kDa

Gene ID: 3006

NCBI Accession: XP_010816821