BRD3 antibody (C-Term)

Catalog No. ABIN2668232

Cited in 1 publication. The Rabbit Polyclonal anti-BRD3 antibody (ABIN2668232) specifically detects BRD3 in WB, ChIP-seq, ChIP and CUT&RUN. The antibody is reactive with Human and Mouse samples.

Quick Overview for BRD3 antibody (C-Term) (ABIN2668232)

Target: BRD3 (Bromodomain Containing 3 (BRD3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRD3 antibody is un-conjugated

Application: Western Blotting (WB), ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)

Product Details anti-BRD3 Antibody

Binding Specificity: C-Term

Purpose: BRD3 antibody (pAb)

Purification: Affinity Purified

Immunogen: This antibody was raised against a peptide within the C-terminal region of mouse BRD3.

Isotype: IgG

Application Details

Application Notes: ChIP: 10 µL per ChIP ChIP-Seq & ChIP-chip: 10 µL each WB: 1:500- 1:2,000 dilution CUT&RUN: 1 µL per 50 µL reaction

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-BRD3 antibody (ABIN2668232)

Sartor, Powell, Brothers, Wahlestedt: "Epigenetic Readers of Lysine Acetylation Regulate Cocaine-Induced Plasticity." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 45, pp. 15062-72, (2015) (PubMed).

Target Details for BRD3

Target: BRD3 (Bromodomain Containing 3 (BRD3))

Alternative Name: BRD3

Background: BRD3 (Bromodomain Containing 3) is a member of the mammalian BET (Bromodomain Extra Terminal) family of proteins, binds hyperacetylated chromatin and has been reported to interact with acetylated Histone H4K5, Histone H4K12 and Histone H3K14. It plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors. Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity). Regulates transcription of the CCND1 gene.

Molecular Weight: 100 kDa

Gene ID: 67382

NCBI Accession: NP_001107046

Pathways: Chromatin Binding