RAD21 antibody (N-Term)

Catalog No. ABIN2668243

The Mouse Monoclonal anti-RAD21 antibody is suitable to detect RAD21 in samples from Human and Mouse. It has been validated for WB, IF and ICC.

Quick Overview for RAD21 antibody (N-Term) (ABIN2668243)

Target: RAD21 (RAD21 Homolog (RAD21))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAD21 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 52A311

Product Details anti-RAD21 Antibody

Binding Specificity: N-Term

Purpose: Rad21 antibody (mAb) (Clone 52A311)

Purification: Protein G Chromatography

Immunogen: This Rad21 antibody was raised against recombinant protein corresponding to the N-terminal half of mouse Rad21.

Isotype: IgG

Application Details

Application Notes: ICC/IF: 0.5 μg/mL dilution WB: 0.5 - 2 μg/mL dilution This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91245.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for RAD21

Target: RAD21 (RAD21 Homolog (RAD21))

Alternative Name: Rad21

Background: Rad21 Clone 52A311 (HR21, MCD1, NXP1, hHR21) is highly similar to the gene product of S. pombe Rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. Rad21 is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of Rad21 with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.

Molecular Weight: 100 kDa

Gene ID: 5885

NCBI Accession: NP_033035

Pathways: Positive Regulation of Endopeptidase Activity