EIF2C3 antibody (AA 1-175)

Catalog No. ABIN2668249

There are 2+ publications for this product available. The Mouse Monoclonal anti-EIF2C3 antibody is suitable to detect EIF2C3 in samples from Human. It has been validated for IF, ICC, IP and ChIP.

Quick Overview for EIF2C3 antibody (AA 1-175) (ABIN2668249)

Target: EIF2C3 (Eukaryotic Translation Initiation Factor 2C3 (EIF2C3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EIF2C3 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Clone: 4B1-F6

Product Details anti-EIF2C3 Antibody

Binding Specificity: AA 1-175

Purpose: Ago3 antibody (mAb) (Clone 4B1-F6)

Purification: Protein G Chromatography

Immunogen: This Ago3 (Argonaute 3) antibody was raised against a recombinant protein corresponding to amino acids 1-175 of human Ago3.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-EIF2C3 antibody (ABIN2668249)

Shang, Zhang, Xu, Xi, Zhang, Wang, Wu: "Ribozyme-enhanced single-stranded Ago2-processed interfering RNA triggers efficient gene silencing with fewer off-target effects." in: Nature communications, Vol. 6, pp. 8430, (2015) (PubMed).

Azuma-Mukai, Oguri, Mituyama, Qian, Asai, Siomi, Siomi: "Characterization of endogenous human Argonautes and their miRNA partners in RNA silencing." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 23, pp. 7964-9, (2008) (PubMed).

Target Details for EIF2C3

Target: EIF2C3 (Eukaryotic Translation Initiation Factor 2C3 (EIF2C3))

Alternative Name: Ago3

Background: Argonaute 3 (Ago3, aka EIF2C3) is a member of the Argonaute family of proteins involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Ago3, however, lacks RNase activity and does not appear to cleave target mRNA molecules. Ago3 has both a PAZ and a PIWI domain, indicative of the Argonaute family of proteins.

Molecular Weight: 110 kDa

Gene ID: 192669

NCBI Accession: NP_079128

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway