DNMT3B antibody (AA 1-218)

Catalog No. ABIN2668253

Cited in 1 publication. The Rabbit Polyclonal anti-DNMT3B antibody (ABIN2668253) specifically detects DNMT3B in WB. The antibody is reactive with Mouse samples.

Quick Overview for DNMT3B antibody (AA 1-218) (ABIN2668253)

Target: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNMT3B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DNMT3B Antibody

Binding Specificity: AA 1-218

Purpose: DNMT3B antibody (pAb)

Purification: Affinity Purified

Immunogen: This DNMT3B antibody was raised against a recombinant protein corresponding to amino acids 1-218 of mouse DNMT3B.

Isotype: IgG

Application Details

Application Notes: WB*: 1:500 - 1:1,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-DNMT3B antibody (ABIN2668253)

Ajj, Chesnel, Pinel, Plenat, Flament, Dumond: "An alkylphenol mix promotes seminoma derived cell proliferation through an ERalpha36-mediated mechanism." in: PLoS ONE, Vol. 8, Issue 4, pp. e61758, (2013) (PubMed).

Target Details for DNMT3B

Target: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))

Alternative Name: DNMT3B

Background: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 Beta) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2 and DNMT3. The DNMT3 family contains two active methyltransferases, DNMT3A & DNMT3B, and one DNMT3-Like protein (DNMT3L). DNMT3B has been shown to be important in the regulation of specific patterns of DNA methylation, specifically de novo methylation. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression. Mutations in the DNMT3B gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome.

Molecular Weight: 110 kDa

Gene ID: 13436

NCBI Accession: NP_001003960