KDM4A antibody (AA 586-674)

Catalog No. ABIN2668289

The Mouse Monoclonal anti-KDM4A antibody (Clone N154-32) (ABIN2668289) specifically detects KDM4A in WB. The antibody is reactive with Human and Mouse samples.

Quick Overview for KDM4A antibody (AA 586-674) (ABIN2668289)

Target: KDM4A (Lysine (K)-Specific Demethylase 4A (KDM4A))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This KDM4A antibody is un-conjugated

Application: Western Blotting (WB)

Clone: N154-32

Product Details anti-KDM4A Antibody

Binding Specificity: AA 586-674

Purpose: JMJD2A antibody (mAb) (Clone N154/32)

Purification: Protein A Chromatography

Immunogen: This JMJD2A antibody was raised against a recombinant protein corresponding to amino acids 586-674 of human JMJD2A.

Isotype: IgG1

Application Details

Application Notes: WB*: 1 - 2 μg/mL dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. The addition of 0.05 % Tween 20 in the blocking buffer and primary antibody incubation buffer is also recommended to aid in detection by Western blot. This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91143.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for KDM4A

Target: KDM4A (Lysine (K)-Specific Demethylase 4A (KDM4A))

Alternative Name: JMJD2A

Background: JMJD2A (KDM4A, JHDM3A) is a member of the Jmj-containing (Jumonji) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. JMJD2A specifically demethylates histone H3 dimethyl lysine 9 (H3K9me2), trimethyl lysine 9, (H3K9me3), dimethyl lysine 36 (H3K36me2) and trimethyl lysine 36 (H3K36me3). It also participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1. JMJD2A contains JmjC and JmjN domains, two PHD fingers and two tudor domains.

Molecular Weight: 130 kDa

Gene ID: 9682

NCBI Accession: NP_055478

Pathways: Warburg Effect