Histone H2A, H4 (H4S1ph) antibody

Catalog No. ABIN2668297

The Rabbit Polyclonal anti-Histone H2A, H4 antibody is suitable to detect Histone H2A, H4 in samples from Human. It has been validated for WB and DB.

Quick Overview for Histone H2A, H4 (H4S1ph) antibody (ABIN2668297)

Target: Histone H2A, H4

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Dot Blot (DB)

Product Details

Binding Specificity: H4S1ph

Purpose: Histone H2A/H4S1ph antibody (pAb)

Purification: Unpurified

Immunogen: This Histone H2A/H4 phospho Ser1 antibody was raised against a peptide including phospho-serine 1 of human histone H2A.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details

Target: Histone H2A, H4

Alternative Name: Histone H2A/H4

Background: Histones H2A and H4 are core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. The first five amino acids of histone H2A and histone H4 are identical in metazoans and include an amino-terminal serine residue. Histone H2A phosphorylation at serine 1 is enriched during mitosis, while H4 phosphorylation increases subsequent to DNA double-strand breaks.

Molecular Weight: 14 and 8 kDa

Gene ID: 92815