Histone H2A, Idic Patch (AA 89-91) antibody

Catalog No. ABIN2668306

There are 4+ publications for this product available. The Rabbit Polyclonal anti-Histone H2A, Idic Patch antibody is suitable to detect Histone H2A, Idic Patch in samples from Human. It has been validated for ChIP and WB.

Quick Overview for Histone H2A, Idic Patch (AA 89-91) antibody (ABIN2668306)

Target: Histone H2A, Idic Patch

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Application: Chromatin Immunoprecipitation (ChIP), Western Blotting (WB)

Product Details

Binding Specificity: AA 89-91

Purpose: Histone H2A, acidic patch antibody (pAb)

Purification: Unpurified

Immunogen: This Histone H2A, acidic patch antibody was raised against a peptide including amino acids 89-91 of histone H2A.

Application Details

Application Notes: ChIP: 10 µL per ChIP WB*: 1:500 - 1:2,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References

Lodhi, Kossenkov, Tulin: "Bookmarking promoters in mitotic chromatin: poly(ADP-ribose)polymerase-1 as an epigenetic mark." in: Nucleic acids research, Vol. 42, Issue 11, pp. 7028-38, (2014) (PubMed).

Alvarez-Saavedra, De Repentigny, Lagali, Raghu Ram, Yan, Hashem, Ivanochko, Huh, Yang, Mears, Todd, Corcoran, Bassett, Tokarew, Kokavec, Majumder, Ioshikhes, Wallace, Kothary, Meshorer, Stopka et al.: "Snf2h-mediated chromatin organization and histone H1 dynamics govern cerebellar morphogenesis and neural maturation. ..." in: Nature communications, Vol. 5, pp. 4181, (2014) (PubMed).

Armour, Bennett, Braun, Zhang, McMahon, Gygi, Harper, Sinclair: "A high-confidence interaction map identifies SIRT1 as a mediator of acetylation of USP22 and the SAGA coactivator complex." in: Molecular and cellular biology, Vol. 33, Issue 8, pp. 1487-502, (2013) (PubMed).

Benatti, Dolfini, Viganò, Ravo, Weisz, Imbriano: "Specific inhibition of NF-Y subunits triggers different cell proliferation defects." in: Nucleic acids research, Vol. 39, Issue 13, pp. 5356-68, (2011) (PubMed).

Target Details

Target: Histone H2A, Idic Patch

Background: Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression.

Molecular Weight: 14 kDa

Gene ID: 92815

NCBI Accession: NP_003508