CHAF1A antibody

Catalog No. ABIN2668347

This Hamster Monoclonal antibody specifically detects CHAF1A in WB, IF, IP and ICC. It exhibits reactivity toward Human. It has been mentioned in 3+ publications

Quick Overview for CHAF1A antibody (ABIN2668347)

Target: CHAF1A (Chromatin Assembly Factor 1, Subunit A (p150) (CHAF1A))

Reactivity: Human

Host: Hamster

Clonality: Monoclonal

Conjugate: This CHAF1A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-CHAF1A Antibody

Purification: Protein G Chromatography

Immunogen: The CAF-1 p150 antobody was raised against full-length CAF-1 purified from human 293 cells.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References for anti-CHAF1A antibody (ABIN2668347)

Hoek, Stillman: "Chromatin assembly factor 1 is essential and couples chromatin assembly to DNA replication in vivo." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 21, pp. 12183-8, (2003) (PubMed).

Kaufman, Kobayashi, Kessler, Stillman: "The p150 and p60 subunits of chromatin assembly factor I: a molecular link between newly synthesized histones and DNA replication." in: Cell, Vol. 81, Issue 7, pp. 1105-14, (1995) (PubMed).

Smith, Stillman: "Immunological characterization of chromatin assembly factor I, a human cell factor required for chromatin assembly during DNA replication in vitro." in: The Journal of biological chemistry, Vol. 266, Issue 18, pp. 12041-7, (1991) (PubMed).

Target Details for CHAF1A

Target: CHAF1A (Chromatin Assembly Factor 1, Subunit A (p150) (CHAF1A))

Alternative Name: CAF-1 p150

Background: CAF-1 p150 (Chromatin assembly factor-1 p150 subunit, CAF1A, CHAF1A) is the largest subunit of the CAF-1 complex that is required for the assembly of nucleosomes onto newly replicated DNA. Specifically, CAF-1 assists in the loading of the H3-H4 tetramer and may be involved in DNA damage repair.

Molecular Weight: 150 kDa

Gene ID: 10036