CENPA antibody (pSer18)

Catalog No. ABIN2668362

This Rabbit Polyclonal antibody specifically detects CENPA in WB, IF, ICC and DB. It exhibits reactivity toward Human.It has been mentioned in 1 publication

Quick Overview for CENPA antibody (pSer18) (ABIN2668362)

Target: CENPA (Centromere Protein A (CENPA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CENPA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Dot Blot (DB)

Product Details anti-CENPA Antibody

Binding Specificity: pSer18

Purpose: CENP-A phospho Ser18 antibody (pAb)

Purification: Affinity Purified

Immunogen: This antibody was raised against a synthetic peptide containing phosphorylated serine 16 and 18 of human CENP-A.

Isotype: IgG

Application Details

Application Notes: ICC/IF: 1:100 dilution WB: 1:100 - 1:500 dilution The addition of 0.05 % Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-CENPA antibody (ABIN2668362)

Logsdon, Barrey, Bassett, DeNizio, Guo, Panchenko, Dawicki-McKenna, Heun, Black: "Both tails and the centromere targeting domain of CENP-A are required for centromere establishment." in: The Journal of cell biology, Vol. 208, Issue 5, pp. 521-31, (2015) (PubMed).

Target Details for CENPA

Target: CENPA (Centromere Protein A (CENPA))

Alternative Name: CENP-A

Background: CENP-A (Centromere Protein A) is a Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. It is required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. Recently, phosphorylation of CENP-A on Ser16 and Ser18 was reported and found to be important for chromosome segregation during mitosis.

Molecular Weight: 16 kDa

Gene ID: 1058

NCBI Accession: NP_001800

Pathways: Chromatin Binding, Maintenance of Protein Location