Histone 3 antibody (H3K56ac)

Catalog No. ABIN2668375

There are 3+ publications for this product available. The Mouse Monoclonal anti-Histone 3 antibody is suitable to detect Histone 3 in samples from Human, Saccharomyces cerevisiae and Schizosaccharomyces pombe. It has been validated for ChIP and DB.

Quick Overview for Histone 3 antibody (H3K56ac) (ABIN2668375)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human, Saccharomyces cerevisiae, Schizosaccharomyces pombe

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Chromatin Immunoprecipitation (ChIP), Dot Blot (DB)

Clone: 12-1

Product Details anti-Histone 3 Antibody

Binding Specificity: H3K56ac

Purpose: Histone H3K56ac antibody (mAb) (Clone 12.1)

Purification: Protein A Chromatography

Immunogen: This Histone H3 acetyl Lys56 antibody was raised against a peptide containing acetyl-Lys56 of human histone H3.

Isotype: IgG1

Application Details

Application Notes: ChIP: 2 - 10 μg per ChIP DB: 1 μg/mL This antibody has not been validated for use in Western blotting. For a Histone H3K56ac antibody recommended for Western blot, see Catalog No. 39281. This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91127.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Histone 3 antibody (ABIN2668375)

Zhang, Li, Rezaeian, Xu, Chou, Jin, Han, Pan, Wang, Long, Zhang, Huang, Tsai, Tsai, Logothetis, Lin: "H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis." in: Nature communications, Vol. 8, pp. 14799, (2017) (PubMed).

Tessarz, Santos-Rosa, Robson, Sylvestersen, Nelson, Nielsen, Kouzarides: "Glutamine methylation in histone H2A is an RNA-polymerase-I-dedicated modification." in: Nature, Vol. 505, Issue 7484, pp. 564-8, (2014) (PubMed).

Drogaris, Villeneuve, Pomiès, Lee, Bourdeau, Bonneil, Ferbeyre, Verreault, Thibault: "Histone deacetylase inhibitors globally enhance h3/h4 tail acetylation without affecting h3 lysine 56 acetylation." in: Scientific reports, Vol. 2, pp. 220, (2012) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histone H3 occurs at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as CBP/p300. Acetylation of histones is often associated with transcriptional activation. Histone H3 Lys56 acetylation occurs normally during S phase, but disappears in G2. This modification persists in presence of DNA damage and also plays a role in nucleosome assembly. Rtt109 was shown to be the major histone acetyltransferase (HAT) for Lys56 acetylation.

Molecular Weight: 17 kDa

Gene ID: 3020

NCBI Accession: NP_003522