Histone 3 antibody (H3K9ac)

Catalog No. ABIN2668378

There are 3+ publications for this product available. The Mouse Monoclonal anti-Histone 3 antibody is suitable to detect Histone 3 in samples from Human and Mouse. It has been validated for WB, IF, ChIP, ICC, DB and ChIP-seq.

Quick Overview for Histone 3 antibody (H3K9ac) (ABIN2668378)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

Clone: 1B10

Product Details anti-Histone 3 Antibody

Binding Specificity: H3K9ac

Purpose: Histone H3K9ac antibody (mAb) (Clone 1B10)

Purification: Protein A Chromatography

Immunogen: The Histone H3 acetyl Lys9 antibody was raised against a peptide including acetyl-lysine 9 of human Histone H3.

Isotype: IgG3

Application Details

Application Notes: ChIP: 2 - 5 μg per ChIP ChIP-Seq: 5 μg each WB: 0.2 - 2 μg/mL dilution DB: 0.2 - 2 μg/mL dilution CUT&Tag: 1 μg per 50 µL reaction NGS-QC certification: this antibody has been processed by the NGS-QC generator. For additional details, click here. For Histone H3K9ac, we also offer AbFlex Histone H3K9ac Recombinant Antibody (rAb). For details, see Catalog No. 91103.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in 63 mM Trisodium Citrate, 70 mM Sodium Phosphate Tribasic, 301 mM Sodium Chloride, 30 % Glycerol, and 0.035 % Sodium Azide. Sodium Azide is highly toxic. *Due to the high levels of NA+ in the antibody storage buffer, precipitation might be observed. Incubate the antibody tube at 37 °C and mix prior to use.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Histone 3 antibody (ABIN2668378)

Tran, Nguyen, Takabe, Yamagata, Miyake: "Pre-treatment with amitriptyline causes epigenetic up-regulation of neuroprotection-associated genes and has anti-apoptotic effects in mouse neuronal cells." in: Neurotoxicology and teratology, Vol. 62, pp. 1-12, (2017) (PubMed).

Mitsuda, Shimizu: "Epigenetic Repeat-Induced Gene Silencing in the Chromosomal and Extrachromosomal Contexts in Human Cells." in: PLoS ONE, Vol. 11, Issue 8, pp. e0161288, (2016) (PubMed).

Sanders, Hagood, Liu, Zhang, Ambalavanan, Thannickal: "Histone deacetylase inhibition promotes fibroblast apoptosis and ameliorates pulmonary fibrosis in mice." in: The European respiratory journal, Vol. 43, Issue 5, pp. 1448-58, (2014) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background: Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. Acetylation of Lys9 is associated with trascriptional activation of genes.

Molecular Weight: 17

Gene ID: 8352

NCBI Accession: NP_003522