Histone 3 antibody (H3K9me2)

Catalog No. ABIN2668395

This Mouse Monoclonal antibody specifically detects Histone 3 in WB, IF, ChIP, ICC and DB. It exhibits reactivity toward Human. It has been mentioned in 3+ publications

Quick Overview for Histone 3 antibody (H3K9me2) (ABIN2668395)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB)

Clone: MABI 0307

Product Details anti-Histone 3 Antibody

Binding Specificity: H3K9me2

Purpose: Histone H3K9me2 antibody (mAb) (Clone MABI 0307)

Purification: Protein G Chromatography

Immunogen: This Histone H3 dimethyl Lys9 antibody was raised against a synthetic peptide containing dimethyl Lys9 of human histone H3.

Isotype: IgG1

Application Details

Application Notes: Validated Applications: ChIP: 10 μg per ChIP WB: 0.5 - 2 μg/mL dilution ICC/IF: 1 - 2 µL/mL dilution Published Applications: ChIP WB See references for more information. Individual optimization may be required.

Restrictions: For Research Use only

Handling

Concentration: 0.38 μg/μL

Buffer: PBS pH 7.5 containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Fluorophore-conjugated antibodies should be protected from light,to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing,Fluorophore- & enzyme-conjugated antibodies should be stored at 4 °C

Storage: 4 °C,-20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage, to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Expiry Date: 24 months

References for anti-Histone 3 antibody (ABIN2668395)

Mitsuda, Shimizu: "Epigenetic Repeat-Induced Gene Silencing in the Chromosomal and Extrachromosomal Contexts in Human Cells." in: PLoS ONE, Vol. 11, Issue 8, pp. e0161288, (2016) (PubMed).

Valdez, Li, Murray, Ji, Liu, Popat, Champlin, Andersson: "Comparison of the cytotoxicity of cladribine and clofarabine when combined with fludarabine and busulfan in AML cells: Enhancement of cytotoxicity with epigenetic modulators." in: Experimental hematology, Vol. 43, Issue 6, pp. 448-61.e2, (2015) (PubMed).

Gregory, Cheung: "Natural variation in the histone demethylase, KDM4C, influences expression levels of specific genes including those that affect cell growth." in: Genome research, Vol. 24, Issue 1, pp. 52-63, (2014) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 9 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SuvH39H1 or G9a. This methylated lysine can be demethylated by histone demethylases as JMJD1A, LSD1 or JMJD2C. Methylation of this residue is mainly associated with transcriptional repression.

Molecular Weight: 17 kDa

Gene ID: 3020

NCBI Accession: NP_003522