Histone 3 antibody (H3K27ac)

Catalog No. ABIN2668396

There are 5+ publications for this product available. The Mouse Monoclonal anti-Histone 3 antibody is suitable to detect Histone 3 in samples from Human. It has been validated for WB, IF, ChIP, ICC, DB and ChIP-seq.

Quick Overview for Histone 3 antibody (H3K27ac) (ABIN2668396)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

Clone: MABI 0309

Product Details anti-Histone 3 Antibody

Binding Specificity: H3K27ac

Purpose: Histone H3K27ac antibody (mAb) (Clone MABI 0309)

Purification: Protein G Chromatography

Immunogen: This Histone H3 acetyl Lys27 antibody was raised against a synthetic peptide including acetyl-lysine 27 of human histone H3.

Isotype: IgG1

Application Details

Application Notes: ChIP: 5 - 10 μg per ChIP ChIP-Seq: 5 - 10 μg each ICC/IF: 1 - 2 μg/mL dilution WB: 0.5 - 2 µL/mL dilution CUT&Tag: 1 μg per 50 µL reaction ChIP-Seq validation was performed by Active Motif's Epigenetics Services, the complete data set is available in the UCSC Genome Browser by clicking here. NGS-QC certification: this antibody has been processed by the NGS-QC generator. For additional details, click here. For Histone H3K27ac, we also offer AbFlex Histone H3K27ac Recombinant Antibody (rAb). For details, see Catalog No. 91193.

Restrictions: For Research Use only

Handling

Concentration: 0.43 μg/μL

Buffer: PBS pH 7.5 containing 30 % glycerol. 0.3M NaCl, and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Histone 3 antibody (ABIN2668396)

Dheekollu, Wiedmer, Sentana-Lledo, Cassel, Messick, Lieberman: "HCF1 and OCT2 Cooperate with EBNA1 To Enhance OriP-Dependent Transcription and Episome Maintenance of Latent Epstein-Barr Virus." in: Journal of virology, Vol. 90, Issue 11, pp. 5353-67, (2016) (PubMed).

Kotomura, Harada, Ishihara: "The Proportion of Chromatin Graded between Closed and Open States Determines the Level of Transcripts Derived from Distinct Promoters in the CYP19 Gene." in: PLoS ONE, Vol. 10, Issue 5, pp. e0128282, (2015) (PubMed).

Gong, Qian, Ertl, Astle, Wang, Harrison, Xu: "Histone modifications change with age, dietary restriction and rapamycin treatment in mouse brain." in: Oncotarget, Vol. 6, Issue 18, pp. 15882-90, (2015) (PubMed).

Chang, Huang, Han, Jiang, Fang, Song, Padilla, Wang, Qu, Stamatoyannopoulos, Li, Papayannopoulou et al.: "Transcriptional environment and chromatin architecture interplay dictates globin expression patterns of heterospecific hybrids derived from undifferentiated human embryonic stem cells or from their ..." in: Experimental hematology, Vol. 41, Issue 11, pp. 967-979.e6, (2013) (PubMed).

Kimura, Hayashi-Takanaka, Goto, Takizawa, Nozaki: "The organization of histone H3 modifications as revealed by a panel of specific monoclonal antibodies." in: Cell structure and function, Vol. 33, Issue 1, pp. 61-73, (2008) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression.Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histone H3 occurs at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as CBP/p300. Acetylation of histone H3 at Lys27 is associated with transcriptional activation.Histone H3K27 can also be mono-, di- or trimethylated by different histone methyltransferases, such as EZH2 or NSD3. While histone methylation can be associated with transcriptional activation or repression, methylation of Lysine 27 of histone H3 is mainly associated with transcriptional repression.

Molecular Weight: 17 kDa

Gene ID: 3020

NCBI Accession: NP_003522