Histone 3 antibody (meLys27)

Catalog No. ABIN2668399

There are 3+ publications for this product available. The Mouse Monoclonal anti-Histone 3 antibody is suitable to detect Histone 3 in samples from Human. It has been validated for WB, IF, ChIP, ICC, DB, ChIP-seq and CUT&Tag.

Quick Overview for Histone 3 antibody (meLys27) (ABIN2668399)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Tagmentation (CUT&Tag)

Clone: MABI 0321

Product Details anti-Histone 3 Antibody

Binding Specificity: meLys27

Purpose: Histone H3K27me1 antibody (mAb) (Clone MABI 0321)

Purification: Protein G Chromatography

Immunogen: This Histone H3 monomethyl Lys27 antibody was raised against a peptide containing monomethyl Lys27 of human Histone H3.

Isotype: IgG2a

Application Details

Application Notes: ChIP: 2 - 5 μg per ChIP ChIP-Seq: 2 - 5 μg each WB: 0.5 - 2 μg/mL dilution CUT&Tag: 1-2 μg per 50 µL reaction ChIP-Seq validation was performed by Active Motif's Epigenetics Services, the complete data set is available in the UCSC Genome Browser by clicking here.

Restrictions: For Research Use only

Handling

Concentration: 0.51 μg/μL

Buffer: PBS pH 7.5 containing 30 % glycerol. 0.3M NaCl, and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Histone 3 antibody (ABIN2668399)

Jacob, Bergamin, Donoghue, Mongeon, LeBlanc, Voigt, Underwood, Brunzelle, Michaels, Reinberg, Couture, Martienssen: "Selective methylation of histone H3 variant H3.1 regulates heterochromatin replication." in: Science (New York, N.Y.), Vol. 343, Issue 6176, pp. 1249-53, (2014) (PubMed).

Ntziachristos, Tsirigos, Welstead, Trimarchi, Bakogianni, Xu, Loizou, Holmfeldt, Strikoudis, King, Mullenders, Becksfort, Nedjic, Paietta, Tallman, Rowe, Tonon, Satoh, Kruidenier, Prinjha, Akira et al.: "Contrasting roles of histone 3 lysine 27 demethylases in acute lymphoblastic leukaemia. ..." in: Nature, Vol. 514, Issue 7523, pp. 513-7, (2014) (PubMed).

Kimura, Hayashi-Takanaka, Goto, Takizawa, Nozaki: "The organization of histone H3 modifications as revealed by a panel of specific monoclonal antibodies." in: Cell structure and function, Vol. 33, Issue 1, pp. 61-73, (2008) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 27 of histone H3 can be mono-, di- or trimethylated (Histone H3 monomethyl Lys27, Histone H3 dimethyl Lys27 or Histone H3 trimethyl Lys27) by different histone methyltransferases such as EZH2 or NSD3. Methylation of this residue is mainly associated with transcriptional repression.

Molecular Weight: 17 kDa

Gene ID: 3020

NCBI Accession: NP_003522