Histone 3 antibody (H3K27me3)

Catalog No. ABIN2668400

Cited in 3+ publications. The Mouse Monoclonal anti-Histone 3 antibody (Clone MABI 0323) (ABIN2668400) specifically detects Histone 3 in WB, IF, ChIP, ICC, DB and ChIP-seq. The antibody is reactive with Human and Mouse samples.

Quick Overview for Histone 3 antibody (H3K27me3) (ABIN2668400)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

Clone: MABI 0323

Product Details anti-Histone 3 Antibody

Binding Specificity: H3K27me3

Purpose: Histone H3K27me3 antibody (mAb) (Clone MABI 0323)

Purification: Protein G Chromatography

Immunogen: This Histone H3 trimethyl Lys27 antibody was raised against a peptide containing trimethyl Lys27 of human Histone H3.

Isotype: IgG1

Application Details

Application Notes: WB: 0.5 - 2 μg/mL dilution DB: 0.5 - 2 μg/mL dilution CUT&Tag: 1 μg per 50 µL reaction

Restrictions: For Research Use only

Handling

Concentration: 0.46 μg/μL

Buffer: PBS pH 7.5 containing 30 % glycerol. 0.3M NaCl, and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Histone 3 antibody (ABIN2668400)

Kelsey, Yang, Leung, Minks, Dixon-McDougall, Baldry, Bogutz, Lefebvre, Brown: "Impact of flanking chromosomal sequences on localization and silencing by the human non-coding RNA XIST." in: Genome biology, Vol. 16, pp. 208, (2015) (PubMed).

Herzog, Lempradl, Trupke, Okulski, Altmutter, Ruge, Boidol, Kubicek, Schmauss, Aumayr, Ruf, Pospisilik, Dimond, Senergin, Vargas, Simon, Ringrose: "A strand-specific switch in noncoding transcription switches the function of a Polycomb/Trithorax response element." in: Nature genetics, Vol. 46, Issue 9, pp. 973-81, (2014) (PubMed).

Cooper, Dienstbier, Hassan, Schermelleh, Sharif, Blackledge, De Marco, Elderkin, Koseki, Klose, Heger, Brockdorff: "Targeting polycomb to pericentric heterochromatin in embryonic stem cells reveals a role for H2AK119u1 in PRC2 recruitment." in: Cell reports, Vol. 7, Issue 5, pp. 1456-1470, (2014) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 27 of histone H3 can be mono-, di- or trimethylated (Histone H3 monomethyl Lys27, Histone H3 dimethyl Lys27 or Histone H3 trimethyl Lys27) by different histone methyltransferases such as EZH2 or NSD3. Methylation of this residue is mainly associated with transcriptional repression.

Molecular Weight: 17 kDa

Gene ID: 3020

NCBI Accession: NP_003522