Histone 3 antibody (H3R8me2a)

Catalog No. ABIN2668406

Cited in 2+ publications. The Rabbit Polyclonal anti-Histone 3 antibody (ABIN2668406) specifically detects Histone 3 in WB, ChIP, DB and ChIP-seq. The antibody is reactive with Human and Mouse samples.

Quick Overview for Histone 3 antibody (H3R8me2a) (ABIN2668406)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

Product Details anti-Histone 3 Antibody

Binding Specificity: H3R8me2a

Purpose: Histone H3R8me2a (asymmetric) antibody (pAb)

Purification: Affinity Purified

Immunogen: This Histone H3 dimethyl Arg8 asymmetric antibody was raised against a peptide containing dimethyl-Arg8 (asymmetric) of human histone H3.

Isotype: IgG

Application Details

Application Notes: ChIP: 10 µL per ChIP ChIP-Seq: 10 µL each WB: 1:500 dilution ChIP-Seq validation was performed by Active Motif's Epigenetics Services, the complete data set is available in the UCSC Genome Browser by clicking here.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Histone 3 antibody (ABIN2668406)

Papazyan, Voronina, Chapman, Luperchio, Gilbert, Meier, Mackintosh, Shabanowitz, Tackett, Reddy, Coyne, Hunt, Liu, Taverna: "Methylation of histone H3K23 blocks DNA damage in pericentric heterochromatin during meiosis." in: eLife, Vol. 3, pp. e02996, (2014) (PubMed).

Blythe, Cha, Tadjuidje, Heasman, Klein: "beta-Catenin primes organizer gene expression by recruiting a histone H3 arginine 8 methyltransferase, Prmt2." in: Developmental cell, Vol. 19, Issue 2, pp. 220-31, (2010) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background: Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone H3 Arg8, methylated by PRMT enzymes, is involved in nuclear-receptor-mediated transcriptional activation.

Molecular Weight: 17 kDa

Gene ID: 3020

NCBI Accession: NP_003522